@article{204486,
  keywords = {deep mutational scanning, evolution, influenza virus, polymerase},
  author = {Yuan Li and Sarah Arcos and Kimberly Sabsay and Aartjan Velthuis and Adam Lauring},
  title = {Deep mutational scanning reveals the functional constraints and evolutionary potential of the influenza A virus PB1 protein.},
  abstract = {<p>The influenza virus polymerase is important for adaptation to new hosts and, as a determinant of mutation rate, for the process of adaptation itself. We performed a deep mutational scan of the polymerase basic 1 (PB1) protein to gain insights into the structural and functional constraints on the influenza RNA-dependent RNA polymerase. We find that PB1 is highly constrained at specific sites that are only moderately predicted by the global structure or larger domain. We identified a number of beneficial mutations, many of which have been shown to be functionally important or observed in influenza virus{\textquoteright} natural evolution. Overall, our atlas of PB1 mutations and their fitness impacts serves as an important resource for future studies of influenza replication and evolution.</p>},
  year = {2023},
  journal = {Journal of virology},
  volume = {97},
  pages = {e0132923},
  month = {12/2023},
  issn = {1098-5514},
  doi = {10.1128/jvi.01329-23},
  language = {eng},
}